| 產(chǎn)品編號 | bs-4271R |
| 英文名稱 | ARHGAP20 Rabbit pAb |
| 中文名稱 | Rho GTP酶激活蛋白20抗體 |
| 別 名 | Arhgap20; KIAA1391; RARHOGAP; RHG20_HUMAN; Rho GTPase activating protein 20; Rho GTPase-activating protein 20; Rho type GTPase activating protein 20; Rho-type GTPase-activating protein 20. |
| 研究領域 | 腫瘤 細胞生物 信號轉(zhuǎn)導 細胞周期蛋白 G蛋白偶聯(lián)受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 133 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ARHGAP20: 1001-1191/1191 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Tissue Specificity: Expressed predominantly in the brain. Lower expression is found in lymph nodes. Similarity: Contains 1 PH domain. Contains 1 Ras-associating domain. Contains 1 Rho-GAP domain. SWISS: Q9P2F6 Gene ID: 57569 Database links: Entrez Gene: 57569 Human Omim: 609568 Human SwissProt: Q9P2F6 Human Unigene: 6136 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ARHGAP20) Polyclonal Antibody, Unconjugated (bs-4271R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
