| 產(chǎn)品編號 | bs-4625R |
| 英文名稱 | CK12 Rabbit pAb |
| 中文名稱 | 細胞角蛋白12抗體 |
| 別 名 | 65 kDa cytokeratin; CK 12; CK 3; CK12; CK3; Cytokeratin 12; Cytokeratin 3; K12; K3; keratin 12(Meesmann corneal dystrophy); Keratin 12; Keratin 3; Keratin, type I cytoskeletal 12; K1C12_HUMAN; Keratin, type II cytoskeletal 3; KRT12; KRT3. |
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Specific References (3) | bs-4625R has been referenced in 3 publications.
[IF=4.658] Fan NW et al. Pigment epithelium-derived factor peptide promotes limbal stem cell proliferation through hedgehog pathway. J Cell Mol Med. 2019 Jul;23(7):4759-4769. IHF ; Mouse.
[IF=3.464] Xiao Yichen. et al. Galectin-3 Is a Crucial Immunological Disease Marker in Patients with Fungal Keratitis. DIS MARKERS. 2022;2022:1380560 IF ; Human.
[IF=3.315] Li Z et al. Rapid Differentiation of Multi-Zone Ocular Cells from Human Induced Pluripotent Stem Cells and Generation of Corneal Epithelial and Endothelial Cells. Stem Cells Dev. 2019 Mar 5. IF ; Human.
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| 研究領域 | 細胞生物 信號轉導 細胞類型標志物 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Dog) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞外基質 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CK12/Cytokeratin 12: 151-250/494 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cytokeratin 12 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with family member KRT12. Cytokeratin 12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Defects in KRT3 and KRT12 are a cause of Meesmann corneal dystrophy (MCD), an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Symptoms occur in adulthood and include rupture of the corneal microcysts that may lead to photophobia, contact lens intolerance and intermittent diminution of visual acuity. Defects in KRT12 are a cause of juvenile epithelial corneal dystrophy of Meesmann (MCD) Function: May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity). Subunit: Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12. Tissue Specificity: Cornea specific. DISEASE: Corneal dystrophy, Meesmann (MECD) [MIM:122100]: An autosomal dominant corneal disease characterized by fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the intermediate filament family. SWISS: Q99456 Gene ID: 3859 Database links: Entrez Gene: 3859 Human Entrez Gene: 268482 Mouse Omim: 601687 Human SwissProt: Q99456 Human SwissProt: Q64291 Mouse Unigene: 66739 Human Unigene: 436651 Mouse Unigene: 94852 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CK12 Polyclonal Antibody, Unconjugated(bs-4625R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
