| 產(chǎn)品編號 | bs-8650R |
| 英文名稱 | T2R38 Rabbit pAb |
| 中文名稱 | 味覺感受器蛋白T2R38抗體 |
| 別 名 | PTC; PTC bitter taste receptor; TAS2R38; T2R38; T2R61; taste receptor, type 2, member 38; T2R38_HUMAN. |
| 研究領域 | 神經(jīng)生物學 通道蛋白 G蛋白偶聯(lián)受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Chicken) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human T2R38/TAS2R38: 151-250/333 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The sense of taste is essential for the survival of organisms. For example, the ability to identify sweet-tasting foods enables animals to seek out food with high nutritive value, whereas the ability to identify bitter substances enables them to avoid the ingestion of potentially harmful substances. A family of integral membrane proteins are involved in taste perception and include T1R, which is involved in sweet taste perception and T2R, which is involved in bitter taste perception. Both types of taste receptors couple to various G proteins to initiate signal transduction cascades. Specifically, T2R38 is expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells. Variations in T2R38 are associated with the ability to taste the bitter chemical phenylthiocarbamide (PTC), also called thiourea tasting. Function: TAS2R38 is gustducin-linked and may play a role in the perception of bitterness and in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells. Similarity: Belongs to the G-protein coupled receptor T2R family. SWISS: P59533 Gene ID: 5726 Database links: Entrez Gene: 5726 Human Omim: 607751 Human SwissProt: P59533 Human Unigene: 647085 Human TAS2R38是一種苦味味覺感受器，該感受器可以嘗出苯硫脲和丙硫氧嘧啶或者相關物質的苦味。這一點和大多數(shù)苦味感受器有所不同，即該感受器是對某類物質敏感，而大多數(shù)感受器則不會表現(xiàn)出強烈的選擇性，能對多種物質做出響應。該感受器所能嘗出的相應物質大量存在于西蘭花、卷心菜以及其它十字花科植物中，例如：葡萄糖異硫氰酸鹽及其相關產(chǎn)物異硫氰酸酯。目前全球人類中，約有75%該感受器相關基因正常，即，可以嘗出苦味。而剩下的25%則無法嘗出這類的苦味，或者敏感度較低。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
