| 產(chǎn)品編號 | bsm-60575R |
| 英文名稱 | CD62L Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | G3B6 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:1000,Flow-Cyt=1:50,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 鈣依賴性凝集素通過與鄰近細胞上的糖蛋白結(jié)合來介導細胞粘附。介導淋巴細胞與外周淋巴結(jié)高內(nèi)皮微靜脈內(nèi)皮細胞的粘附。促進內(nèi)皮細胞中白細胞的初始束縛和滾動。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: Human serum
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 42 kDa
Observed MW: 100 kDa
Cell line: Raji
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60575R
Cell line: EL4
Fixation: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60575R
Cell line: Ramos
Fixation: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60575R
Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60575R
Cell line: PBMC
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60575R
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
