| 產(chǎn)品編號(hào) |
bs-1111R-PE-Cy7 |
| 英文名稱(chēng) |
Rabbit Anti-Oct-3/Oct-4/PE-Cy7 Conjugated antibody
|
| 中文名稱(chēng) |
PE-Cy7標(biāo)記的胚胎干細(xì)胞關(guān)鍵蛋白抗體 |
| 別 名 |
POU domain, class 5, transcription factor 1;Oct-3/4; Oct-4A; Oct4A; Oct 4A; Octamer-binding transcription factor 3; Octamer-4; Octamer-3; Oct-3; NF-A3; Otf-3; Otf3; MGC22487; Oct 3; Oct 4; OCT3; Oct4; Octamer binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding protein 3; Octamer-binding protein 4; OTF 3; OTF 4; OTF-3; OTF3; OTF4; PO5F1_HUMAN; POU class 5 homeobox 1; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU type homeodomain containing DNA binding protein; POU5F1; POU5F1. |
| 規(guī)格價(jià)格 |
100ul/2980元
購(gòu)買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) |
100ul
|
| 研究領(lǐng)域 |
細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號(hào) 干細(xì)胞 表觀遺傳學(xué) |
| 抗體來(lái)源 |
Rabbit |
| 克隆類(lèi)型 |
Polyclonal |
| 交叉反應(yīng) |
Human, Mouse, Rat,
(predicted: Dog, Pig, Cow, )
|
| 產(chǎn)品應(yīng)用 |
IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 |
39kDa |
| 性 狀 |
Lyophilized or Liquid |
| 濃 度 |
1mg/ml |
| 免 疫 原 |
KLH conjugated synthetic peptide derived from human OCT-4 |
| 亞 型 |
IgG |
| 純化方法 |
affinity purified by Protein A |
| 儲(chǔ) 存 液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background:
Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
Function:
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Subunit:
Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2.
Subcellular Location:
Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern.
Tissue Specificity:
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Post-translational modifications:
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
Similarity:
Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Database links:
Entrez Gene: 5460 Human
Entrez Gene: 282316 Cow
Entrez Gene: 18999 Mouse
Entrez Gene: 100127461 Pig
Entrez Gene: 294562 Rat
Omim: 164177 Human
SwissProt: O97552 Cow
SwissProt: Q01860 Human
SwissProt: P20263 Mouse
SwissProt: Q9TSV5 Pig
Unigene: 249184 Human
Unigene: 632482 Human
Unigene: 646545 Human
Unigene: 17031 Mouse
Unigene: 161748 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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